Surface Enhanced Raman Spectroscopic Study of DNA and 6-mercapto-1-hexanol Interactions Using Large Area Mapping

Kasper Bayer Frøhling, Tommy Sonne Alstrøm, Michael Bagge, Michael Stenbæk Schmidt, Mikkel Nørgaard Schmidt, Jan Larsen, Mogens Havsteen Jakobsen, Anja Boisen

AbstractThe emergence of 2D SERS substrates with large areas of hot spots has enabled data to be gathered at large
scale. This work presents a statistical tool for analysing large amounts of SERS data by utilizing a peakfitting
model in a specific spectral range. By analysing the distributions of Raman intensities and peak
positions it is possible to directly inspect the interplay between DNA and 6-mercapto-1-hexanol on gold covered nanopillars. It is demonstrated that optimised functionalization parameters can be extracted from the Raman spectra directly. Using the peak-fitting approach it is possible to avoid miss-interpretation of intensity histograms, where contamination might contribute with an enhanced background and not a peak.
KeywordsBayesin modelling, SERS, area mapping, DNA, 6-mercapto-1-hexanol, thiol, gold, peak-fitting
TypeJournal paper [With referee]
JournalVibrational Spectroscopy
Year2016    Month August
PublisherElsevier
BibTeX data [bibtex]
IMM Group(s)Intelligent Signal Processing


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