Improving the detection limit of an inflammatory protein assay on a micro cantilever and DVD readout system

Michael Bache, Fillipo G. Bosco, Lutoslawa Mikowska, Tommy S. AlstrÝm, En-te Hwu, Ching-Hsiu Chen, Jesper Eugen-Olsen, Ing-Shouh Hwang, Anja Boisen

AbstractThe development of a bioassay using cantilevers is a challenging task. To reach clinical relevant detection levels of a biomarker in whole blood samples is even more so. In the last 3 years we have used a high-throughput nanomechanical detection system based on DVD-ROM optical drive and cantilever sensors to detect the presence of soluble urokinase Plasminogen Activator Receptor inflammatory biomarker (suPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody-antigen recognition was investigated as a function of suPAR concentration.
TypeConference paper [With referee]
SeriesProceedings of the 11th Nanomechanical Sensing Workshop
BibTeX data [bibtex]
IMM Group(s)Intelligent Signal Processing

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