Determination of myosin filament positions and orientations in electron micrographs of muscle cross sections |
Bjarni Bodvarsson, Soren Klim, Stig Mortensen, Martin Morkebjerg, James Chen, Julian R. Maclaren, Chun H. Yoon, Pradeep K. Luther, John M. Squire, Andrew Bainbridge-Smith, Philip J. Bones, Rick P. Millane
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| Abstract | An automated image analysis system for determination of myosin filament orientations in electron micrographs of muscle cross-sections is described. Analysis of the distribution of the orientations is important in studies of muscle structure, particularly for interpretation of x-ray diffraction data. Filament positions are determined using h-dome extraction and image filtering, based on grayscale reconstruction. Erroneous locations are eliminated based on lattice regularity. Filament orientations are determined by correlation with a template that incorporates the salient filament characteristics and classified using a Gaussian mixture model. Application to a number of micrographs and comparison with manual classifications of orientations shows that the system is effective in many cases. |
| Type | Conference paper [With referee] |
| Conference | Proc. SPIE Int. Soc. Opt. Eng. |
| Year | 2004 Month October Vol. 5562 pp. 97-109 |
| Note | doi:10.1117/12.562357 |
| Publication link | http://spiedl.aip.org/getabs/servlet/GetabsServlet?prog=normal&id=PSISDG005562000001000097000001&idtype=cvips&gifs=Yes |
| BibTeX data | [bibtex] |
| IMM Group(s) | Image Analysis & Computer Graphics |