@CONFERENCE\{IMM2007-05224,
    author       = "Y. Kato and D. A. Holm and B. Okollie and D. Artemov",
    title        = "Visualization of Drug Distribution in Brain Tumors in Mice by {MRSI}",
    year         = "2007",
    month        = "apr",
    booktitle    = "Proc 98th American Association for Cancer Research Annual Meeting",
    volume       = "",
    series       = "",
    editor       = "",
    publisher    = "",
    organization = "",
    address      = "",
    url          = "http://www2.compute.dtu.dk/pubdb/pubs/5224-full.html",
    abstract     = "Purpose: In mouse brain tumor models, little 				 uptake of high molecular weight (MW) {MR} contrast agents is detected due to blood-brain barrier (BBB) and blood-brain tumor barrier (BTB).  We have tested i) a combination of bolus tracking and dynamic contrast enhanced (DCE) {MRI} following injection of low {MW} contrast agent, GdDTPA, to extract vascular parameters of the tumor, and ii) indirect detection 13C magnetic resonance spectroscopic imaging (MRSI) in brain tumor-bearing mice following injection of 13C-labeled anticancer agent, temozolomide [13C]TMZ.
Methods: For {DCE} and bolus tracking experiments, Saturation-recovery T1 (TE/TR=1.245/500ms, {TD}=0.25, 0.{5,} 1s, slice thickness=2mm) and {FLASH} T2* (TE/TR=3.5/500ms, slice thickness=2mm) sequences were acquired.  For inverse-detection {MRSI} experiments, heteronuclear multiple-quantum coherence (HMQC) technique (TE/TR=15/1500ms, 8\&\#61620;8 voxels, slice thickness=4mm) was used with {WALTZ-}16 decoupling in 13C channel.  Prior to {MRI}/{MRSI} experiments with mice bearing orthotopic U87MG human glioblastoma xenografts, the mouse tail vein was catheterized for injection of GdDTPA (1:2 dilution of Magnevist®) and an intraperitoneal catheter was inserted for i.p. administration of  [13C]TMZ.  Experimental parameters were optimized with a methanol phantom.  All {MR} studies were carried out on a horizontal bore Bruker Biospec 9.{4T} spectrometer.  {MR} data were analyzed using in-house software written in the {IDL}™ and MatLab™ programming environment."
}