@CONFERENCE\{IMM2004-04998, author = "B. Bodvarsson and S. Klim and S. Mortensen and M. Morkebjerg and J. Chen and J. R. Maclaren and C. H. Yoon and P. K. Luther and J. M. Squire and A. Bainbridge-Smith and P. J. Bones and R. P. Millane", title = "Determination of myosin filament positions and orientations in electron micrographs of muscle cross sections", year = "2004", month = "oct", pages = "97-109", booktitle = "Proc. {SPIE} Int. Soc. Opt. Eng.", volume = "5562", series = "", editor = "", publisher = "", organization = "", address = "", note = "doi:10.1117/12.562357", url = "http://spiedl.aip.org/getabs/servlet/GetabsServlet?prog=normal&id=PSISDG005562000001000097000001&idtype=cvips&gifs=Yes", abstract = "An automated image analysis system for determination of myosin filament orientations in electron micrographs of muscle cross-sections is described. Analysis of the distribution of the orientations is important in studies of muscle structure, particularly for interpretation of x-ray diffraction data. Filament positions are determined using h-dome extraction and image filtering, based on grayscale reconstruction. Erroneous locations are eliminated based on lattice regularity. Filament orientations are determined by correlation with a template that incorporates the salient filament characteristics and classified using a Gaussian mixture model. Application to a number of micrographs and comparison with manual classifications of orientations shows that the system is effective in many cases." }